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Osama Al-Dhubiea

PhD Candidate in Applied Microbiology

Algiers, Algeria

Summary

PhD candidate in Applied Microbiology at the University of Algiers 1, specializing in CRISPR-guided modular engineering of therapeutic microbial chassis for immune reprogramming, with a primary focus on Lactiplantibacillus plantarum WCFS1. Research interests include synthetic biology, microbial genome editing, and the development of next-generation live biotherapeutics.

Email: o.aldhubiea@univ-alger.dzPhone: 0558719599

Experience

Graduate Researcher (Master's Thesis) — Al-Azhar University, Cairo, Egypt

10/2022 — 06/2024

Investigated lipid production potential of local fungal isolates obtained from Yemeni agricultural soils and Egyptian organic wastes. Isolated, purified, and screened 128 fungal strains, identifying three high-yielding isolates (Rhizopus oryzae UC 13, Aspergillus terreus 1Z, and Aspergillus fumigatus 5H). Optimized fermentation parameters and evaluated lipid profiles using GC-MS analysis. Registered the strains in GenBank and demonstrated the feasibility of using rice straw as a low-cost carbon source for polyunsaturated fatty acid production.

Education

PhD in Applied Microbiology — University of Algiers 1 - Benyoucef Benkhedda

09/2024 — Present

Master of Science/Microbiology — Al-Azhar University, Cairo, Egypt

10/2022 — 06/2024

Master of Science (M.Sc.) in Microbiology — Swami Ramanand Teerth Marathwada University (SRTM University), Nanded, Maharashtra, India

09/2017 — 08/2018

Bachelor of Science (B.Sc.) in Microbiology — Faculty of Science, Sana'a University, Sana'a, Yemen

09/2011 — 10/2015

English Language Diploma — International American Yemeni Institute for Modern Sciences and Human Development, Hodaidah, Yemen.

11/2009 — 09/2010

High School Diploma (Scientific Section) — Al-Sha'ab Basic and Secondary School, Hodaidah, Yemen.

09/2007 — 06/2008

Skills

Microbial isolation and identificationFungal fermentation and optimizationLipid extraction and analysis (GC-MS)Molecular techniques (DNA extractionPCR18S rDNA sequencing)CRISPR-Cas genome editing (theoretical & preparatory knowledge)Synthetic biology and microbial chassis engineeringScientific writing and data analysisLaboratory techniques (aseptic workculturingmicroscopy)

Languages

Arabicand English

Certifications

Solidarty for the Future - Scientific Research Cannot Wait Webinar Series — World Intellectual Property Organization (WIPO) (09/2026)

welc.wipo.int/lms/verify/index.php?q=MUHCofqJDy

OPEN English for Tourism Professionals MOOC — Online Professional English Network (OPEN), U.S. Department of State/ FHI 360 (10/2025)

badges.parchment.com/public/assertions/5LU5LPSxT16D2RrTAPrwSw

Methods for Preparing Academic Theses — The Training, Research, and Studies Center of the Council of Arab Economic Unity, in partnership with the Permanent Mission of Yemen to the League of Arab States (05/2025)

Certificate of Participation: International Seminar on "Climate Resilient Crops for Feeding the Future" (CRCFF-2027) — Organized by Swami Ramanand Teerth Marathwada University, in collaboration with Washington State University, Kansas State University, and USAID. (12/2017)

Certificate of Attendance: ASM Workshop on The Culture of Responsibility: Biological Safety — American Society for Microbiology (ASM) in collaboration with Faculty of Science, Sana'a University. (05/2014)

Projects

Biosynthesis of Some Economically ImportantCompounds from Some Fungal Isolates - Al-Azhar University — Master’s Thesis Project | 2021 – 2024

• Isolated, purified, and screened oleaginous fungal strains from diverse ecological sites in Yemen and Egypt to evaluate lipid accumulation capabilities. • Identified potent lipid-producing strains using morphological characteristics and confirmed findings via molecular identification (18S rDNA sequencing). • Optimized culture and fermentation conditions to enhance lipid yield and discovered novel microbial strains and lipid products. • Developed a cost-effective production bioprocess utilizing rice straw as an inexpensive carbon source to minimize biotechnological operational costs. • Conducted comprehensive fatty acid profiling using Gas Chromatography-Mass Spectrometry (GC-MS) to analyze saturated and unsaturated fatty acid compositions.

share.google/dR70XoNEEd1GPJOKt

CRISPR-Guided Modular Engineering of Therapeutic Microbial Chassis for Immune Reprogramming — PhD Research Focus | 2024– Present

Designing a research framework for engineering Lactiplantibacillus plantarum WCFS1 as a therapeutic platform for immune modulation using CRISPR tools.

Volunteer Experience

State Blood Transfusion Council (SBTC) — Volunteer Blood Donor

10/2018 — 10/2018

Voluntarily participated in a community health drive organized by the State Blood Transfusion Council (SBTC) to support local healthcare services and promote public health awareness.

Awards & Distinctions

Yemeni Government Full PhD Scholarship (Cultural Exchange Program) — Ministry of Higher Education and Scientific Research - Yemen (2024/2025-2027/2028)

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